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زیست شناسی::
ژن پلیهدرین
Consequently, it was reasoned that replacement of the polyhedrin gene with a coding sequence for a het erologous protein, followed by infection of cultured insect cells, would result in the production of large amounts of the heterologous protein.
The gene of interest is inserted into the multiple cloning site (MCS) that lies between the polyhedrin gene promoter (Pp) and polyhedrin gene transcrip tion termination (Pt) sequences.
(B) Replacement of the AcMNPV polyhedrin gene with an expression unit from a transfer vector.
the polyhedrin gene has been deleted from this segment of DNA.
Within some of the doubly transfected cells, a doublecrossover recombination event occurs at homo logous sequences on the transfer vector and in the AcMNPV genome, and the cloned gene with polyhedrin promoter and termination regions becomes integrated into the AcMNPV DNA (Fig. 3.35B) with the concomitant loss of the polyhedrin gene.
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